By suppressing the anti-tumour immune response, the Ultraviolet (UV) wavelengths contained in sunlight are a major contributor to the development and progression of skin cancer. Systemic UV-immunosuppression can also offer protection from the development of autoimmune diseases, most prominently seen in multiple sclerosis. Following exposure to an immune suppressive dose of UV, a large number of cellular and molecular events are triggered that converge on the activation of regulatory cells. As such, UV is a powerful modulator of adaptive immune responses and can influence the induction of tolerance. However, while the mechanism by which UV mediates suppression of local, cutaneous immunity are relatively well understood, how UV suppresses immunity in distant tissues is not. The liver is a particularly important site for T cell inactivation and maintenance of peripheral tolerance. Whether UV affects cell populations in the liver has never been explored before. Multi-parameter flow cytometry was performed on single cell suspensions of livers isolated from mice exposed, or not, to the skin with an immune suppressive dose of UV. A significant increase in CD3^hiNK1.1^hi cells was observed in mice exposed to UV, confirming that NKT cells are a target of UV. This study highlights the liver as a particularly important organ affected by, and possibly involved in, UV-induced immune suppression.